Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays
نویسندگان
چکیده
منابع مشابه
Protein profiling in cancer cell lines and tumor tissue using reverse phase protein arrays
Reverse phase protein array (RPPA) technology is an antibody-based high-throughput assay for protein profiling of biological specimens that allows for many measurements with very small amounts of cell lysate. Here, we report the sensitivity, reproducibility, and accuracy of a particular RPPA platform called Zeptosens. We customized the RPPA protocol for our in-house setup, and measured more t...
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Protein microarrays offer a new means by which to conduct quantitative profiling of disease-associated proteins. The knowledge gained may provide novel strategies for early detection, diagnosis and therapeutic intervention. A variety of sophisticated approaches, including gene arrays, sequencing consortiums and large-scale two-dimensional gel electrophoresis, continue to generate lists of prote...
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Protein microarrays offer a new means by which to conduct quantitative profiling of diseaseassociated proteins. The knowledge gained may provide novel strategies for early detection, diagnosis and therapeutic intervention. A variety of sophisticated approaches, including gene arrays, sequencing consortiums and large-scale two-dimensional gel electrophoresis, continue to generate lists of protei...
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Recent advances in high throughput, high content "omic" technologies coupled with clinical information has lead to the expectation that the complexity of the molecular information generated will lead to more robust scientific research as well as the expectation that overarching therapeutic approaches will be patient-tailored to the underlying specific molecular defects of the disease. As diseas...
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ژورنال
عنوان ژورنال: Cancer Cell
سال: 2017
ISSN: 1535-6108
DOI: 10.1016/j.ccell.2017.01.005